|[ Ana Sayfa | Editörler | Danışma Kurulu | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | E-Posta ]|
|Fırat Tıp Dergisi|
|2001, Cilt 2, Sayı 3, Sayfa(lar) 209-215|
|[ Turkish ]|
|The Effects of Ritodrine and Ritodrine-Indomethacin Combination on Spontaneous Contractions of Myometrium Isolated From Pregnant Rats in Which Preterm Labour was Induced by Lipopolysaccharide Administration|
|Hüsnü ÇELİK1, Ahmet AYAR2, Ahmet KALKAN3, Kibar ÇEBİ1|
|1Fırat Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, ELAZIĞ
2Fırat Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı,
3Fırat Üniversitesi, Tıp Fakültesi, İnfeksion Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı
The aim of this study was to determine and compare the efficiency of ritodrine and ritodrine-indomethacin combination on spontaneous contractions of myometrium isolated from pregnant rats which presents Lipopolysaccharide (LPS)-induced preterm labour model. 50µ.g/kg LPS (Escherichia coli, serotype 055:B5, Sigma) was intraperitonealy injected and myometrium samples obtained after servical dislocation and isometric contractions were evaluated. Results were statistically analysed using Wilcoxon Rank, Kruskal-Wallis Analysis of Variance and Mann Whitney U tests. Application of 10-8 g/ml ritodrine inhibited the amplitude and frequency of spontaneous contractions of myometrium isolated from normal pregnant rats by 96.6% and 69.8%, respectively CP<0.05).
The same dose of ritodrine was also applied to the myometrium isolated from LPS-induced preterm model rats, the inhibition on amplitude and frequency of contractions was %85.5 and %16, respectively (P <0.05). When combination of 10-8g/ml ritodrine + 1µg/ml indomethacin was applied the inhibitory effects on amplitude and frequency of contractions was increased in myometrium isolated from LPS administered rats (97.2%, 46.5%, P <0.05) but no significant change was observed in spontaneous contractions of myometrium obtained from normal pregnant rats compared to application of indomethacin alone (P >0.05 ). The results from this study indicate that combination with low dose of indomethacin may improve the decreased efficiency of ritodrine in infection related preterm labour.
|[ Turkish ]|