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Fırat Tıp Dergisi
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Investigation of the Effect of Daily Oral Vitamin C in Preventing the Postoperative Adhesion Formation: An Experimental Study
Şehmus PALA1, Remzi ATILGAN1, Tuncay KULOĞLU2, Melike BAŞPINAR1, Emre YALÇIN1, Şeyda YAVUZKIR1
1Fırat Üniversitesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Elazığ, Türkiye
2Fırat Üniversitesi, Histoloji ve Embriyoloji Anabilim Dalı, Elazığ, Türkiye

Objective: Investigation of the effect of daily oral vitamin C in preventing the adhesion on uterine horn model in rats.

Material and Method: Twenty one female Wistar Albino rats were divided into three groups as random. Group 1 (n =7): Only abdomen was opened and closed. Group 2 (n =7): A 2 cm linear incision was made to the right uterine horn and closed with 4/0 vicryl. Group 3 (n =7): After the administra-tion of vitamin C at a dose of 50 mg/kg a day before the operation, a 2 cm linear incision was made to the right uterine horn and closed with 4/0 vicryl. All rats were opened after 15 days. Adhesion scoring and severity were evaluated according to Lisky scale. The right uterine horn was removed and fixed under 10% formaldehyde. Masson’s trichrome, TUNEL and Immuno-histochemical staining were performed on 5 μm sections taken from paraffin blocks. Vascular endothelial growth factor (VEGF), angiogenesis, Type I collagen and malondialdehyde (MDA) were performed histologi-cally in the traumatized uterine horn region.

Results: Adhesion area and adhesion severity were significantly lower in G3 than G2. When G2 and G1 were compared, fibrosis, angiogenesis, VEGF, Type 1 collagen and MDA scores were significantly higher in G2. VEGF and type 1 collagen score were significantly higher in G3 than G1. Fibrosis, angiogenesis and MDA scores were significantly higher in G2 than in G3.

Conclusion: Daily oral vitamin C was found to be effective in preventing intraabdominal adhesion formation in the rat uterin horn model.


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