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Fırat Tıp Dergisi
2004, Cilt 9, Sayı 4, Sayfa(lar) 103-107
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Determination of Paraoxonase Activity in Halothane Induced Hepatotoxicity
Ömer Lütfi ERHAN1, A. Belin ÖZER1, Ferit GÜRSU2, Fethi YILMAZ3, Necati TİMURKAAN3, Funda GÜLCÜ2, Kenan GÜLBAYRAK1
1 Fırat Üniversitesi Tıp Fakültesi Anesteziyoloji ve Reanimasyon Anabilim Dalı, ELAZIĞ
2Fırat Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı, ELAZIĞ
3 Fırat Üniversitesi Veteriner Fakültesi Patoloji Anabilim Dalı, ELAZIĞ

Objective: %20 of halothane is metabolized in endoplasmic reticulum of hepatocyte by oxidation and reduction. Paraoxonase is a natural antioxidative enzyme either found in plasma and not in liver as a high level. The aim of the study is expressing of biochemical and histopathologic relation between effects of paraoxonase which synthesized and released by liver and halothane on liver.

Materials and Methods: In this study, 70 rats which ranged between 180-200 gr used. 10 of them divided as control group. In the study % 1.5 halothane applied for 2 hours in first (60 rats), third (40 rats) and fifth (20 rats) day. 10 rats sacrified before and after for each application than blood and liver sampling performed. Paraoxonase, and malonil dialdehide levels measured in this blood samples. This blood samples are also examined as histopathologically.

Results and Conclusions: Significant and opposite relation were found between paraoxonase levels (169.63 ± 49.07), histopathologic damage, MDA (0.583±0.059) and other enzyme levels (p<0.05) in the studay groups. While histopathologic damage increases with repetative application, paraoxonase (110.71±9.70) level decreases. In the later period paraoxonase level (192.12±70.12) will increase and degree of damage will decrease (p<0.05).

Conclusions: The indicator of suppression of protein synthesis by halothane in liver is that decreasing of paraoxonase synthesis while application. In the later period histopathologic results will show opposite relation with this enhancement. In the literature there is no relationship between halothane and paraoxonase because of this new studies are needed for expression of pathologic changes. ©2004, Fırat Üniversitesi, Tıp Fakültesi

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