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Fırat Tıp Dergisi
2007, Cilt 12, Sayı 4, Sayfa(lar) 246-250
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Histological and Histochemical Studies on the Effect of Single Dose of Cyclophosphamide on Migration of Primordial Germ Cells of Fetal Charles Foster Rat – A Preliminary Study
Biswabina RAY, Bhagath Kumar POTU
Kasturba Medical College, Anatomy, manipal, KARNATAKA, INDIA

Objectives: Effect of single dose Cyclophosphamide (CP), a known teratogen on early ovarian development was studied to understand whether it affects the primordial germ cells (PGC) in migratory phase.

Materials and Methods: Adult female pregnant Charles foster rats were divided into control and experimental groups. Experimental group was given intraperitoneal injection of CP at a dose of 2mg/kg of body weight on day 10 of gestation when PGCs were in migratory phase. 12 fetuses from each group were sacrificed on 16th day of gestation to process for histochemical studies (alkaline phosphatase staining), whereas the ovaries of 12 fetuses from each group were collected on 20th day of gestation for histological studies (haematoxyline and eosin staining).

Results: Examination of sections of the fetuses of group I showed clusters of large circular PGCs showing blackish brown color in 16 day old fetus. In experimental group, only homogeneous light brown cells were present. Ovaries of 20 day old fetus showed ovarian follicles with large germ cells in the centre in controls. Germ cells were totally absent in treated group.

Conclusion: It was concluded that the CP, inhibited migration of PGCs to gonadal ridge leading to absence in ovary. ©2007, Fırat University, Medical Faculty


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