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Fırat Tıp Dergisi
2008, Cilt 13, Sayı 1, Sayfa(lar) 009-014
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An Experimental Study On Histopathological Effects Of Melatonin On Cyclosporin Induced Heart Damage
Meltem KURUŞ1, Mukaddes EŞREFOĞLU1, Engin ŞAHNA2, Sedat SEVİL3, Ali OTLU1
1İnönü Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji Anabilim Dalı, MALATYA
2Fırat Üniversitesi Tıp Fakültesi Farmakoloi Anabilim Dalı, ELAZIĞ
3İnönü Üniversitesi Tıp Fakültesi Anatomi Anabilim Dalı, MALATYA

Objectives: In recent studies, the damage of tissues caused by a Cyclosporine A (CyA) and the role of free oxygen radicals in this damage have been discussed widely. Melatonin, the most important indoleamine released from the pineal gland, is a free radical scavenger and antioxidant. In our study, histopathological investigation of the possible beneficial effects of melatonin on the cardiac damage caused by CyA was aimed.

Materials and Methods: This study included 4 groups: Group 1: the control, Group 2: 4 mg/kg/day of melatonin intraperitoneal (i.p.), Group 3: 2 mg/kg/day of CyA subcutaneously (s.c.), and Group 4: 4 mg/kg/day of Melatonin i.p. plus 2 mg/kg/day of CyA s.c. administered group. Our study lasted for 28 days for each group. At the end of this period the rats were decapitated under terminal anesthesia. Their hearts were removed and embedded in paraffin blocks for sectioning. .At the end of the treatments cardiac tissue was treated for morphological (Hematoxylin and Eosin) analyses.

Results: The sections of the control and melatonin groups were normal histologically. The myocard and the network among the myocardial tissue were uneven in the CyA administered group. In CyA administered group the localizations of the nuclei of the myocardial cells were not central. In some areas, the unity of the cells came to be damaged, and disintegration and vacuolization of the cytoplasm could be observed. Abundant cell infiltration was recognized in some areas. There was connective tissue increase from place to place and fibrosis could be observed especially around the cardiac fibrils and vessel walls. Another histopathoogical finding was the existence of small haemorrhagic fields.In the CyA plus Melatonin group, it was determined that all these histopathological findings were significantly diminished.

Conclusion: As a result of this study, it has been concluded that the damage due to CyA is reversible and can be lowered by the administration of Melatonin.. ©2008, Firat University, Medical Faculty

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