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Fırat Tıp Dergisi
2008, Cilt 13, Sayı 3, Sayfa(lar) 162-166
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Anti-Apoptotic Actions of Omega-3 Fatty Acids on Testes in Experimental Formaldehyde Intoxication: An Immunohistochemical Study
İlter KUŞ1, İsmail ZARARSIZ2, Nusret AKPOLAT3, Murat ÖGETÜRK1, Murat A. KUŞ4, Oğuz Aslan ÖZEN4, Mustafa SARSILMAZ1
1Fırat Üniversitesi Tıp Fakültesi Anatomi Anabilim Dalı, ELAZIĞ
2Mustafa Kemal Üniversitesi Tayfur Ata Sökmen Tıp Fakültesi, Anatomi Anabilim Dalı, HATAY
3Fırat Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı, ELAZIĞ
4Afyon Kocatepe Üniversitesi Tıp Fakültesi, Anatomi Anabilim Dalı, AFYON

Objective: In this study, which we have performed immunohistochemically, it was aimed to investigate effects of formaldehyde toxicity on the testes, especially on apoptotic changes. Potential antioxidant effects of omega-3 fatty acids against these toxic effects were also evaluated.

Materials and Methods: A total of 21 adult male Wistar-albino rats used in our study were divided into three equal groups. Rats in group I were used as control. Rats in group II were injected intraperitoneally with formaldehyde every other day while the other ones received omega-3 fatty acids daily via intragastric gavage (Marincap capsule®) with injections of formaldehyde. At the end of two-week experimental period, all animals were killed by decapitation and their testes were removed. For light microscopic examination, tissue specimens were embedded in paraffin blocks following routine histological procedures. Section obtained from paraffin blocks stained with Bax protein immunohistochemically for the determination of apoptosis. The intensity of immunohistochemical Bax stain within cell cytoplasm was scored semi-quantitatively. Results: it was found an intensive immunohistochemical Bax staining in tissue specimens of formaldehyde-exposed rats, while no staining was seen in tissue sections of control testes. On the other hand, immunohistochemical Bax staining was observed minimally in tissue sections of testes of rats administered with formaldehyde plus omega-3 fatty acids.

Conclusion: According to results of this study performed immunohistochemically, omega-3 fatty acids prevent apoptosis caused by formaldehyde in the testes. ©2008, Firat University, Medical Faculty


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