Objective: To examine the effect of applying 3%, 50%, 75% and 95% concentrations of trichloroacetic acid by attachment and instillation methods for endometrial ablation on ovarian reserve and dysplasic changes in ovarian surface epithelium in rats.

Material and Method: Ninety rats were included in a randomized, bloc-designed, concentration-matched study program. Group1: (n=10): Abdomen was opened and closed. The groups in which 3% (Group 2, n=10), 50% (Group 3, n=10), 75% (Group 4, n=10), and 95% (Group 5, n=10) TCA attachment was applied, after abdomen was opened and a 2 cm. lengthwise incision was made in the left uterine horn. The groups in which 0,1 ml of 3% (Group 6, n=10), 50% (Group 7, n=10), 75% (Group 8, n=10) and 95% (Group 9, n=10) TCA instillation was applied to the left uterine horn, after the abdomen was opened. Abdomens of all rats were opened 21 days later. Left oophorectomy was performed. Preparations were evaluated in terms of ovarian follicle reserve and changes in ovarian surface epithelium. Group 2 and Group 6, Group 3 and Group 7, Group 4 and Group 8, Group 5 and Group 9 were compared (MWU test). Kruskall Wallis variance analysis was employed in the comparison including all groups (p<0.05 significant).

Results: Ovarian reserve and changes in surface epithelium were found similar in instillation and attachment groups (p>0.05). The comparison including all groups revealed that ovarian follicle reserve and surface epithelium changes were similar (p>0.05).

Conclusion: Application of various concentrations of TCA by attachment and instillation methods for endometrial ablation doesn't have any unfavorable effect on ovarian reserve and ovarian surface epithelium in rats.