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Fırat Tıp Dergisi
2012, Cilt 17, Sayı 2, Sayfa(lar) 094-099
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The Effects of Vasodilator Solutions on Apoptosis in Saphenous Vein with Mechanical Distention Application
Rafet TOK1, Ayhan UYSAL2, Oktay BURMA2, İbrahim Hanifi ÖZERCAN3
1Batman Özel Dünya Hastanesi, Kalp ve Damar Cerrahisi Kliniği, Batman, Türkiye
2Fırat Üniversitesi Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Elazığ, Türkiye
3Fırat Üniversitesi Tıp Fakültesi, Patoloji Anabilim Dalı, Elazığ, Türkiye

Objective: This study explores the influence of vasodilators on the apoptotic activity related to endothelial damage caused by mechanical distension of the saphenous vein.

Materials and Methods: Thirty-two patients without varicose veins undergoing coronary artery bypass graft operations were divided into four equal groups. Group I (control) Normal Saline, group II Papaverine group III Verapamil and group IV Nitroglycerine solutions. Percentange of immunohistochemical reactivity, pro-apoptotic bax and kaspaz-9 and anti-apoptotic bcl-2 were measured in the samples.

Results: The percentage of cells which were positive for Bax (+) In Group I (Control) was 85.00 ± 6.89, Group II (Papaverine) 64.63 ± 8.73, Group III (Verapamil) 23.75 ± 4.20 and Group IV (Nitroglycerine) 22.00 ± 7.69. While there was no statistical difference between verepamil and nitroglycerin groups, they both had significantly decreased percentage of cells with proapoptotic Bax (+) compared to the control and the papaverine groups (p<0.001). Percentage of cells that were stained with the antiapoptotic BCL (+) stain were lowest in the control (2.50 ± 1.41) and the Papaverine (2.75 ± 1.28) groups and significantly higher in the Verapamil (7.25 ± 1.83) and Nitroglycerine (8.88 ± 2.30) groups (p<0.001). There was no difference between the groups with respect to Caspase-9 immunoreactivity.

Conclusion: The results of this study suggest that addition of Verapamil and or Nitroglycerine to the solutions that are used in the preparation of the saphenous vein might have a beneficial effect on the reduction of the apoptotic activity.


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