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Fırat Tıp Dergisi
2012, Cilt 17, Sayı 3, Sayfa(lar) 148-152
[ Turkish ] [ Tam Metin ] [ PDF ]
Comparison of Endometrial Tissue Co-cultures in Fertile and Infertile Women with Implantation Failure
Leyla BAHAR1, Semra KAHRAMAN2, Tülin BAYKAL3, Bora REŞİTOĞLU4
1Mersin Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Mersin, Türkiye
2Memorial Hastanesi, Yardımcı Üreme Teknikleri & Genetik Merkezi, İstanbul, Türkiye
3Mersin Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Mersin, Türkiye
4Mersin Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, Mersin, Türkiye

Objective: Failure of implantation is one of the most important factors affecting the success of in vitro fertilization (IVF) applications. In this study, women in fertile and infertile women with previous implantation failure in IVF applications are aimed to be compared within their endometrial cocultures.

Materials and Methods: Ten fertile women and the seventeen women with recurrent implantation failure (RIF) were included to the study, endometrium biopsy samples were taken. Monolayer of co-culture technique was applied to the endometrial samples. In the endometrial co-culture of fertile and RIF group individuals, glands and stromal cells were evaluated by invert microscope and statistical analysis was performed.

Results: Co-cultures of the endometrium of fertile and RIF group women were compared with the microscope. Morphological differences were observed for the development of gland and stromal cells. In the fertile group, development of endometrial co-culture cells was better than RIF group. RIF group co-culture cells, less developed and had less number. Development of RIF group cells was less than quantitative terms. As a result of comparison of gland and stromal cells statistically, in fertile and RIF group, significant difference was observed.

Conclusion: When the endometrial co-cultures of fertile and RIF women were compared, the difference was found significant not only morphologically but also statistically. However, it was stated that the endometrial co-culture applications increase the implantation rates in RIF patients. So new studies are needed to explain the disclosure of molecular mechanisms of implantation and to improve the quality of the embryo development.


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