Objective: In this study we aimed to investigate if Hylan GF-20 and methyl-prednisolone will effect upon the formation of adhesions in rats underwent laparotomy.

Material and Method: Forty rats were divided into 4 groups and abrasion was created with sterile gauze at 1 cm2 area on the anterior wall of the cecum. In the first group, while the rats not given any drug, in the second group 1 cc of 4% solution of Hylan GF-20, in the third group 10 mg / kg methyl prednisolone and in the fourth group 1 cc %4 Hylan GF-20 solüsyonu +10 mg / kg methyl prednisolone 10 mg / kg methyl prednisolone were given intraperitoneally. Seven days later, adhesions with incision line and cecum were evaluated by an investigator blinded to group by doing again laparotomy outside earlier incision line.

Results: The average number of caecal adhesions was, respectively, 1.80 ± 0.92, 0.40 ± 0.70, 0.90 ± 0.74 and 0.80 ± 1.03. The average number of adhesions in the incision line was, respectively, 2.50 ± 1.43, 0.60 ± 0.70, 0.90 ± 0.88 and 0.50 ± 0.71. Adhesion scores of all treatment groups significantly lower than the control group (p <0.05). However, there was no statistically significant difference between treatment groups (p>0.05).

Conclusion: Hylan GF-20 and methyl prednisolone individually are effective in preventing the development of intra-abdominal adhesions. Concomitant use does not increase their effectiveness in the prevention of adhesions.