Material and Method: Ninty-two case sunder went to laparoscopic appendectomy in Elazig Medical Park Hospital from January 2013 to June 2014 was retrospectively screened. Age, gender, hospital stay, operation time, whether complicated appendicitis complicated appendicitis, complications and management of complications were noted. Patients were divided into two groups, Group 1 (hem-o-lok clip) and Group 2 (intra corporeal knot tying). Datas were compared between two groups.

Results: Ninty-twopatients (43 male /49 female) were screened. Mean age was 35.1 (15-77) years. Mean operation time was 28.8 minutes (20-50), mean hospital stay was 1.25 (0-5) days. Operation time was significantly shorter in group 1 (p<0.001), both in uncomplicated (p<0.001) and complicated (p=0.046) cases. Although hem-o-lok group had a low hospital stay and postoperative complication rate, the difference between two groups was not statistically significant.

Conclusion: Hem-o-lok clip is a simple and a safe tool for closure of appendiceal stum with low operation time.

Age, gender, hospital stay, operation time, whether complicated appendicitis or uncomplicated appendicitis, complications (wound infection, mechanical intestinal obstruction and fistula formation) and management of complications were noted prospectively and considered retrospectively. Any complication seen in first postoperative week was accepted as postoperative complication for this study. Patients were divided into two groups according to closure of the stump of appendix: in group 1 it was closed by hem-o-lok clip (Weck Polymer Ligation System ®, medium or large in size) and in group 2 by intracorporeal knot tying. Noted datas were compared between two groups. Additional comparison was performed according to whether the appendicitis was complicated or not. Appendicites was accepted as complicated, if there was one of the following findings; perforation, peritonitis, accompanying abscess formation.

Surgical technique: Patients were operated upon in supine position, which was changed to Trandelenburg and left lateral position when needed. During surgery, after the cleaning of abdominal skin pneumoperitoneum is created by using Veress needle inserted from infraumblical region. Peritoneal space was filled with CO2 at 10mm Hg pressure.10 mm trocar was inserted to abdomen from infraumblical area. Under the guidance of the scope two other 5 mm trocars were inserted from lower abdomen, one of them from suprapubic area and the other from left lower quadrant (Figüre). After traction of the appendix from it's tip, mesoappendix was dissected by ligasure (Covidien Vessel Sealing System ®, 5 mm). Only in cases with a large artery, a titanium clip was applied. The base of the appendix was closed either by application of a large hem-o-lok clip or by intracorporeal knot tying with 00 silk according to the surgeon's preference. After dissection of appendix, it was removed through 10 mm trocar. Peritoneal space was washed with isotonic solution.

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Figure I: Points for insertion of the trocars.

Statistical analysis were performed by using SPSS for Windows 20.0 (SPSS Inc., Chicago, IL, USA). Unpaired t test was used to compare the mean values and Fisher's exact test was used to compare independent parameters. P values of <0.05 were accepted as significant.

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Table I: Comparison of twogroups. p<0.05 accepted as statisticallysignificant (*allpostoperativecomplicationswereseen in caseswithcomplicatedappendicites).

Operation time was significantly longer in knot tying group (p<0.001), especially for uncomplicated cases (p<0.001). Hospital stay was not different in two groups. Although complications were not significantly different, they were seen in more cases in group 2. Wound infections were treated with wound care and oral antibiotic use. Cases with postoperative subileus have not required any special treatment. Fistula was not seen in both groups. All of the seven postoperative complications were seen in cases with complicated appendicitis.

Closure of the stump is an important point in LA due to the potential risk of serious complications such as fistulas, peritonitis and sepsis⁹. Therefore, there have been many defined methods with some superiorities to the others. Operation time, hospital stay and postoperative complications are widely used parameters to compare the benefits of these methods⁹.

Operation time was significantly longer in intracorporeal knot tying group both in complicated and uncomplicated cases. It is not hard to understand that intracorporeal knot tying requires more time when compared to application of a clip. Similarly, Ates et al.² reported a significantly longer operative time in cases of intracorporeal knot tying when compared with titanium clip application. In another study, Colak et al.⁴ reported short operative time with the use of hem-o-lok clip when compared the use of endoloop, but the difference was not statistically significant.

Hospital stay is directly associated with postoperative complications, therefore we detected similar results in these two parameters. Hospital stay and postoperative complication rates were better in hem-o-lok clip group, but it was not statistically significant. However, the p value was close to 0.05 (0.061 for hospital stay and 0.051 for postoperative complications). In two prospective studies that compared the hem-o-lok clip with endoloop, there were no difference between the groups in terms of hospital stay and postoperative complications^4,5. Additionally, Partecke et al.¹⁰ reported that the use of a single hem-o-lok clip is easy to use, safe and cost-effective. They suggested the use of a single clip for the closure of the appendicular stump as the standard procedure in LA. All of the postoperative complications were seen in complicated cases. It can be considered that postoperative complications and hence longer hospital stay were associated with whether the appendicitis was complicated or not, rather than the method for stump closure.

In conclusion, in our opinion, closure of appendix stump with a hem-o-lok clip is a simple and a safe method in LA. Postoperative complications is generally related with presence of perforation, peritonitis or accompanying abscess formation rather than the use of hem-o-lok clip or knot tying.

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