Objective: Acinetobacter baumannii and Pseudomonas aeruginosa are bacteria commonly implicated in hospital-acquired infections and notorious for their ability to rapidly develop antibiotic resistance. This study aimed to assess the antibiotic resistance profiles of Acinetobacter baumannii and Pseudomonas aeruginosa strains isolated from blood cultures collected between 2018 and 2022.

Material and Method: The study encompassed Acinetobacter baumannii and Pseudomonas aeruginosa strains isolated from blood cultures taken between January 2018 and December 2022. BacT/ALERT 3D automated blood culture system was used for blood cultures. Bacterial identification and antibiotic susceptibility tests were performed using both traditional methods and the BD Phoenix 100 automated system.

Results: Over the five-year period, 520 Acinetobacter baumannii and 298 Pseudomonas aeruginosa strains were isolated from blood cultures. Acine-tobacter baumannii accounted for 3.3% of all isolated bacteria, while Pseudomonas aeruginosa represented 1.9%. The majority of Acinetobacter baumannii (92.5%) and Pseudomonas aeruginosa (85.9%) strains were isolated from intensive care units. Acinetobacter baumannii showed high rate resistance to all antibiotics tested. Pseudomonas aeruginosa showed the highest resistance to levofloxacin (63.5%), while the lowest resistance was shown to amikacin (8.1%) and gentamicin (16.8%).

Conclusion: The study determined that Acinetobacter baumannii exhibits alarming levels of antibiotic resistance, while Pseudomonas aeruginosa exhibits a milder resistance profile. These findings emphasize the need to develop effective strategies for the management of hospital infections. It is also important to take precautions such as effective use of available antibiotics and continuous screening and follow-up for the detection of antimicrobial resistance.