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Fırat Tıp Dergisi
2021, Cilt 26, Sayı 4, Sayfa(lar) 211-217
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Investigation of Atypical Pathogenes Using Indirect Immunfluorescan Antibody Method (IFA) in Community-Developed Pneumonies
Recep ÖNCÜ1, Süheyl UÇUCU2, Adnan SEYREK3
1TC Muğla Halk Sağlığı Laboratuvarı Tıbbi Mikrobiyoloji Anabilim Dalı, Muğla, Türkiye
2TC Muğla Halk Sağlığı Laboratuvarı Tıbbi Biyokimya Anabilim Dalı, Muğla, Türkiye
3Fırat Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Elazığ, Türkiye

Objective: The aim of this study is to investigate seropositivity of specific IgM antibodies developed against atypical pneumoniae pathogens and to compare with control group.

Material and Method: Serum samples taken from 90 pediatric patients who were diagnosed with CAP in the 0-14 age group and whose condition was serious enough to require hospitalization and 30 healthy children, a single IFA kit for the most common 20 atypical pathogens (Respiratory Tract Profile IgM, Euroimmune, Lübeck, Germany). The frequency of these factors, the distribution of factors according to age, gender and seasons were determined. This study does not cover pneumonia that develops in patients with congenital or acquired immunodeficiency and patients with underlying chronic diseases.

Results: There were seropositivity at 61 children in patients’ group and 7 children in control group. Sensitivity and specifity of the test were 76,7%. In statistical analyse, seropositivity of patients’ group was significantly higher than control group (p <0,05). In sera of 42 (46,7%) patients İnfluenza virüses, 9 (25,6%) patients other respiratuary virüses, 55 (45,6%) patients enteric virüses particularly Coxackie virüs, from bacteria Bordotella spp. 30 (33,3%), other strains (K. pneumoniae, M. pneumoniae, L. pneumophilia) 16 (17,8%) were positive. In statistical analyze, these positivities were found meaningful to control group (p <0,05). 38 (62,3%) of 61 sera in patients’ group and 4 (57,1%) of 7 seropositive sera in control group, there was seropositivity against multiple agents.

Conclusion: In conclusion, seropositivity in children with CAP was significantly higher than healthy control group and the majority of pathogens were found to be mixed. This result showed that in patients with CAP atypical pathogens may be substantially responsible and the İFA assay, which has high sensitivity and specificity, can be preferred in routine applications with other classic conventional methods to determine the etiology of CAP.

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