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Fırat Tıp Dergisi |
2001, Volume 6, Number 3, Page(s) 442-445 |
[ Turkish ] |
THE EFFICIENCY OF SDS-PAGE METHOD IN DETERMINATION OF F. HEPATICA AND F. GIGANTICA E/S PROTEINS |
Mustafa KAPLAN1, Yasemin BULUT2 |
1Fırat Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, ELAZIĞ 2Fırat Üniversitesi Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, ELAZIĞ |
Aim: The aim of this study was to investigate the efficiency of SDS-PAGE in visualization of described E/S proteins.
Materials and Methods: SDS-PAGE seperation was performed by using 10% seperation gel and 5% stacking gel. Results: About 20 out of the 30 known F. Hepatica E/S proteins and 2 out of 6 F. gigantica E/S proteins were determined. Conclusion: Use of 10% gel in SDS-PAGE method was efficient in determination of F. Hepatica E/S proteins but the success rate was not the same for determination of F. gigantica. |
[ Turkish ] |
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