[ Main Page | Editorial | Advisory Board | About | Table of Contents | Archive | Search | Instructions to Authors | E-Mail ]
Fırat Tıp Dergisi
2001, Volume 6, Number 3, Page(s) 442-445
[ Turkish ]
THE EFFICIENCY OF SDS-PAGE METHOD IN DETERMINATION OF F. HEPATICA AND F. GIGANTICA E/S PROTEINS
Mustafa KAPLAN1, Yasemin BULUT2
1Fırat Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, ELAZIĞ
2Fırat Üniversitesi Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, ELAZIĞ

Aim: The aim of this study was to investigate the efficiency of SDS-PAGE in visualization of described E/S proteins.

Materials and Methods: SDS-PAGE seperation was performed by using 10% seperation gel and 5% stacking gel.

Results: About 20 out of the 30 known F. Hepatica E/S proteins and 2 out of 6 F. gigantica E/S proteins were determined.

Conclusion: Use of 10% gel in SDS-PAGE method was efficient in determination of F. Hepatica E/S proteins but the success rate was not the same for determination of F. gigantica.


[ Turkish ]
[ Main Page | Editorial | Advisory Board | About | Table of Contents | Archive | Search | Instructions to Authors | E-Mail ]