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Fırat Tıp Dergisi
2020, Cilt 25, Sayı 3, Sayfa(lar) 111-116
[ Turkish ] [ Tam Metin ] [ PDF ]
Melatonin May Regulate Apoptotic Pathway Via Affecting Bax, Bcl2I1 and XIAP Levels in Myocardial Ischemia-Reperfusion Injury
Gülnur ASLAN1, Hüseyin Fatih GÜL2, Engin ŞAHNA1, Ebru ÖNALAN3
1Fırat Üniversitesi Tıp Fakültesi, Tıbbi Farmakoloji Anabilim Dalı, Elazığ, Türkiye
2Fırat Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Elazığ, Türkiye
3Fırat Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Elazığ, Türkiye

Objective: Restoring blood flow after ischemia is very important for maintaining the viability of ischemic tissue. When ischemic tissue is reperfused, it causes enzyme degradation, excessive increase in reactive oxygen species and secondary injuries such as apoptosis. Melatonin released from the pineal gland is one of the important endogenous antioxidants. The aim of this study was to investigate the effect of melatonin on proapoptotic B-cell lymphoma 2 associated X (Bax) and antiapoptotic B-cell lymphoma 2I1 (Bcl2I1) and X-linked inhibitor of apoptosis (XIAP) levels by ische-mia/reperfusion (I/R) injury.

Material and Method: Rats were randomly divided into 3 groups as control, I/R, and I/R+melatonin. The left main coronary artery was occluded for 30 min followed by 120 min reperfusion. Melatonin was administrated by intraperitoneal injection during the last 10 days before I/R. Bax, Bcl2I1 and XIAP levels were analyzed by real time-PCR.

Results: The tissue Bax levels increased 1,47 fold while Bcl2I1 and XIAP levels decreased with I/R injury. Melatonin administration showed protec-tion against I/R induced myocardial injury by inhibiting all these changes.

Conclusion: Antiapoptotic XIAP and Bcl2I1 and proapoptotic Bax may be involved in signaling pathways in the pathology of myocardial I/R, and the protective role of melatonin may be due to its antiapoptotic activities.

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