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Fırat Tıp Dergisi
2004, Cilt 9, Sayı 3, Sayfa(lar) 072-074
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Cytogenetic and FISH Analyses of the Abortus Materials: A Useful Approach Towards Genetic Counseling for the Couples with Recurrent Spontaneous Abortions
Haluk AKIN, Hüseyin YÜCE
Fırat University Faculty of Medicine, Deparment of Medical Biology and Genetics, ELAZIĞ

The cytogenetic analyses of the abortus material provide important information about the recurrence risks and possible management for the couples with recurrent spontanous abortions. The methods of cytogenetic analyses depend on primarily cell culture that has relatively higher (10- 40%) failure. Since the most common chromosomal abnormalities in abortus materials are numerical chromosomal abnormalities; we evaluated the compatibility of the karyotypes and interphase-Fluoresan in situ Hybridization (FISH) results and the predictive value of interphase FISH technique in cases with tissue culture failure.

Materials & Methods:
Interphase-FISH slides were prepared using touch preparation protocol from fresh tissue samples to eliminate the collagenase procedure. FISH procedure was carried out using specific probes for centromeric 13, 16, 18 and 21. At least 100 interphase cells for each slides were detected.

Results and Conclusion:
Chromosomal abnormality incidence of our study (45.5%) seems to be compatible with literature. It is founded some numerical aberrations (chromosomal abnormalities) as 18.18%autosomal trisomies, 13.64% triploidies, 4.50% monosomies and 9% mosaicism in cases. Two out of twenty eight abortus materials with tissue culture failure were revealed trisomies by FISH. However, the tissue culture failure incidence of this study (56%) is higher than the reported incidences (10-40%). This shortcoming could be diminished by the improvement of the tissue procurement and transportation conditions for culture. With this approach, supportive data will be accumulated and utilized for future epidemiological studies by concurrent FISH preparations and culture set up. ©2004, Fırat Üniversitesi, Tıp Fakültesi

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